论文
论文题目: Mouse cloning and somatic cell reprogramming using electrofused blastomeres
论文题目英文:
作者: Amjad Riaz1①,赵小阳①,代相鹏①,李伟,刘蕾,万海峰,于洋,王柳,周琪*
论文出处:
年: 2011
卷: 21
期: 5
页: 770-778
联系作者: 周琪
发表期刊: Cell Research
ISSN:
第一作者所在部门:
收录类别:
论文连接
影响因子: 9.417
摘要: Mouse cloning from fertilized eggs can assist development of approaches for the production of "genetically tailored" human embryonic stem (ES) cell lines that are not constrained by the limitations of oocyte availability. However, to date only zygotes have been successfully used as recipients of nuclei from terminally differentiated somatic cell donors leading to ES cell lines. In fertility clinics, embryos of advanced embryonic stages are usually stored for future use, but their ability to support the derivation of ES cell lines via somatic nuclear transfer has not yet been proved. Here, we report that two-cell stage electrofused mouse embryos, arrested in mitosis, can support developmental reprogramming of nuclei from donor cells ranging from blastomeres to somatic cells. Live, full-term cloned pups from embryonic donors, as well as pluripotent ES cell lines from embryonic or somatic donors, were successfully generated from these reconstructed embryos. Advanced stage pre-implantation embryos were unable to develop normally to term after electrofusion and transfer of a somatic cell nucleus, indicating that discarded pre-implantation human embryos could be an important resource for research that minimizes the ethical concerns for human therapeutic cloning. Our approach provides an attractive and practical alternative to therapeutic cloning using donated oocytes for the generation of patient-specific human ES cell lines.

英文摘要:
外单位作者单位: