论文题目: | Androgenetic haploid embryonic stem cells produce live transgenic mice |
论文题目英文: | |
作者: | 李伟①,Ling Shuai①,万海峰①,董明珠①,#Meng Wang,桑励思,冯春敬,#Guan-Zheng Luo,Tianda Li,Xin Li,王立宾,郑钦元,Chao Sheng,#Hua-Jun Wu,#Zhonghua Liu,刘蕾,王柳,#Xiu-Jie Wang,赵小阳*,周琪* |
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年: | 2012 |
卷: | 490 |
期: | 7420 |
页: | 407-411 |
联系作者: | 周琪 |
发表期刊: | Nature |
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论文连接 | http://www.nature.com/nature/journal/vaop/ncurrent/full/nature11435.html |
影响因子: | 36.28 |
摘要: | Haploids and double haploids are important resources for studying recessive traits and have large impacts on crop breeding, but natural haploids are rare in animals. Mammalian haploids are restricted to germline cells and are occasionally found in tumours with massive chromosome loss. Recent success in establishing haploid embryonic stem (ES) cells in medaka fish and mice raised the possibility of using engineered mammalian haploid cells in genetic studies. However, the availability and functional characterization of mammalian haploid ES cells are still limited. Here we show that mouse androgenetic haploid ES (ahES) cell lines can be established by transferring sperm into an enucleated oocyte. The ahES cells maintain haploidy and stable growth over 30 passages, express pluripotent markers, possess the ability to differentiate into all three germ layers in vitro and in vivo, and contribute to germlines of chimaeras when injected into blastocysts. Although epigenetically distinct from sperm cells, the ahES cells can produce viable and fertile progenies after intracytoplasmic injection into mature oocytes. The oocyte-injection procedure can also produce viable transgenic mice from genetically engineered ahES cells. Our findings show the developmental pluripotency of androgenentic haploids and provide a new tool to quickly produce genetic models for recessive traits. They may also shed new light on assisted reproduction. |
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