论文
论文题目: Dermatophytic defensin with antiinfective potential
论文题目英文:
作者: 朱顺义*,高斌,#Peta J. Harvey,#David J. Craik
论文出处:
年: 2012
卷: 102
期: 22
页: 8495-8500
联系作者: 朱顺义
发表期刊: PNAS
ISSN:
第一作者所在部门:
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论文连接 http://www.ncbi.nlm.nih.gov/pubmed/23023130
影响因子: 9.681
摘要:

Fungi are a newly emerging source of peptide antibiotics with therapeutic potential. Here, we report 17 new fungal defensin-like peptide (fDLP) genes and the detailed characterization of a corresponding synthetic fDLP (micasin) from a dermatophyte in terms of its structure, activity and therapeutic potential. NMR analysis showed that synthetic micasin adopts a "hallmark" cysteine-stabilized α-helical and β-sheet fold. It was active on both gram-positive and gram-negative bacteria, and importantly it killed two clinical isolates of methicillin-resistant Staphylococcus aureus and the opportunistic pathogen Pseudomonas aeruginosa at low micromolar concentrations. Micasin killed approximately 100% of treated bacteria within 3 h through a membrane nondisruptive mechanism of action, and showed extremely low hemolysis and high serum stability. Consistent with these functional properties, micasin increases survival in mice infected by the pathogenic bacteria in a peritonitis model. Our work represents a valuable approach to explore novel peptide antibiotics from a large resource of fungal genomes.

英文摘要:
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