| 摘要: |
CRISPRs are clustered, regularly interspaced, short palindromic repeats present in many bacteria and archaea genomes. Proteins encoded by CRISPR-associated (Cas) genes serve as guardians of the genome, which target foreign DNA at specific sites by means of small CRISPR RNA (crRNA)-guided DNA recognition and degradation1, 2, 3, 4. Recently, several groups described how CRISPR-Cas systems efficiently create site-specific gene modifications in whole organisms such asStreptococcus pneumoniae, Escherichia coli, Danio rerio (zebrafish) and mice, suggesting its potential application in the production of genetically engineered organisms5, 6, 7, although germline transmission of the mutations remains to be shown. Here, we report the use of CRISPR-Cas systems to generate multiple gene mutations in rats in a germline-competent manner. |