| 论文题目： | Genetic Modification and Screening in Rat Using Haploid Embryonic Stem Cells |
| 论文题目英文： | |
| 作者： | 李伟①，Xin Li①，李天达①，Ming-Gui Jiang①，万海峰，#Guan-Zheng Luo，冯春敬，崔晓龙，腾飞，Yan Yuan，Quan Zhou，Qi Gu，帅领，#Jia-hao Sha，#Ya-mei Xiao，王柳，#Zhong-hua Liu，#王秀杰，赵小阳，周琪* |
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| 年： | 2013 |
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| 期： | DOI: 10.1016/j.stem.2013.11.016 |
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| 联系作者： | 周琪 |
| 发表期刊： | Cell Stem Cell |
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| 论文连接 | http://www.sciencedirect.com/science/article/pii/S1934590913005079 |
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| 摘要: | The rat is an important animal model in biomedical research, but practical limitations to genetic manipulation have restricted the application of genetic analysis. Here we report the derivation of rat androgenetic haploid embryonic stem cells (RahESCs) as a tool to facilitate such studies. Our approach is based on removal of the maternal pronucleus from zygotes to generate androgenetic embryos followed by derivation of ESCs. The resulting RahESCs have 21 chromosomes, express pluripotency markers, differentiate into three germ layer cells, and contribute to the germline. Homozygous mutations can be introduced by both large-scale gene trapping and precise gene targeting via homologous recombination or the CRISPR-Cas system. RahESCs can also produce fertile rats after intracytoplasmic injection into oocytes and are therefore able to transmit genetic modifications to offspring. Overall, RahESCs represent a practical tool for functional genetic studies and production of transgenic lines in rat. |
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