论文题目: | Cyclin B2 Can Compensate for Cyclin B1 in Oocyte Meiosis I |
论文题目英文: | |
作者: | 李建①,汤济鑫①,程金妹,#Bian Hu,王毓倩,Batool Aalia,李晓宇,靳程,王秀霞,邓守龙,张妍,陈苏仁,#Wei-Ping Qian,孙青原,#Xing-Xu Huang,刘以训* |
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年: | 2018 |
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期: | DOI: 10.1083/jcb.201802077 |
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联系作者: | 刘以训 |
发表期刊: | Journal of Cell Biology |
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论文连接 | http://jcb.rupress.org/content/early/2018/08/09/jcb.201802077.long |
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摘要: | Mammalian oocytes are arrested at the prophase of the first meiotic division for months and even years, depending on species. Meiotic resumption of fully grown oocytes requires activation of M-phase–promoting factor (MPF), which is composed of Cyclin B1 and cyclin-dependent kinase 1 (CDK1). It has long been believed that Cyclin B1 synthesis/accumulation and its interaction with CDK1 is a prerequisite for MPF activation in oocytes. In this study, we revealed that oocyte meiotic resumption occurred in the absence of Cyclin B1. Ccnb1-null oocytes resumed meiosis and extruded the first polar body. Without Cyclin B1, CDK1 could be activated by up-regulated Cyclin B2. Ccnb1 and Ccnb2 double knockout permanently arrested the oocytes at the prophase of the first meiotic division. Oocyte-specific Ccnb1-null female mice were infertile due to failed MPF activity elevation and thus premature interphase-like stage entry in the second meiotic division. These results have revealed a hidden compensatory mechanism between Cyclin B1 and Cyclin B2 in regulating MPF and oocyte meiotic resumption. |
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