李伟，博士，研究员，博士生导师，干细胞与生殖生物学国家重点实验室副主任。2006年获武汉大学理学学士学位，2012年获中国科学院大学理学博士学位。2013年入选中国科学院动物研究所“星辰研究员”计划并建立课题组，2016年评估优秀转任研究员。主要从事基因工程和干细胞等创新生物技术的研发，并利用这些新技术和模型揭示哺乳动物生殖与再生的基础调控规律，开发重大疾病的基因治疗。作为通讯/共同通讯作者在Science、Nature、Cell等刊物发表论文50余篇。获授权发明专利20余项。获得国家杰出青年科学基金、优秀青年科学基金、日本实验动物学会“国际奖”、中国科学院“青年科学家奖”、中国科学院杰出科技成就奖（突出贡献者）、中国科学院朱李月华优秀教师奖等资助和奖励。

　　基因组信息及其表达调控是生命多样性和复杂性的重要基础，也是疾病治疗和健康干预的重要靶标和手段。我们特别感兴趣结合基因工程、细胞工程和合成生物学等手段建立新的基因工程技术和细胞/动物模型，用于（1）揭示生殖与再生相关性状及其种间进化差异的调控机制；（2）设计和制造功能定制化的细胞；（3）重大疾病的基因治疗。

　　主持国家重点研发计划项目、中国科学院稳定支持基础研究领域青年团队计划项目等。

1. Cui T#, Cai B#, Tian Y, Liu X, Liang C, Gao Q, Li B, Ding Y, Li R, Zhou Q, Li W*, Teng F*. Therapeutic In Vivo Gene Editing Achieved by a Hypercompact CRISPR-Cas12f1 System Delivered with All-in-One Adeno-Associated Virus. Advanced Science (Weinh) 2024 Feb 26:e2308095. doi: 10.1002/advs.202308095. Online ahead of print.
2. Wang C#, Fang S#, Chen Y#, Tang N, Jiao G, Hu Y, Li J, Shan Q, Wang X, Feng G, Zhou Q*, Li W*. High-efficiency targeted transgene integration via primed micro-homologues. Cell Discovery 2023 Jul 4;9(1):69. doi: 10.1038/s41421-023-00552-0.
3. Wang LB#, Li ZK#, Wang LY#, Xu K#, Ji TT#, Mao YH, Ma SN, Liu T, Tu CF, Zhao Q, Fan XN, Liu C, Wang LY, Shu YJ, Yang N, Zhou Q*, Li W*. A sustainable mouse karyotype created by programmed chromosome fusion. Science 2022 Aug 26;377(6609):967-975. doi: 10.1126/science.abm1964. Epub 2022 Aug 25.
4. He ZQ#, Li YH#, Feng GH#, Yuan XW#, Lu ZB#, Dai M, Hu YP, Zhang Y, Zhou Q*, Li W*. Pharmacological Perturbation of Mechanical Contractility Enables Robust Transdifferentiation of Human Fibroblasts into Neurons. Advanced Science (Weinh). 2022 May;9(13):e2104682. doi: 10.1002/advs.202104682.
5. Chen Y#, Hu Y#, Wang X#, Luo S, Yang N, Chen Y, Li Z, Zhou Q*, Li W*. Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing. Innovation (Camb). 2022 May 26;3(4):100264. doi: 10.1016/j.xinn.2022.100264.
6. Hu Y#, Chen Y#, Xu J#, Wang X, Luo S, Mao B, Zhou Q*, Li W*. Metagenomic discovery of novel CRISPR-Cas13 systems. Cell Discovery 2022 Oct 11;8(1):107. doi: 10.1038/s41421-022-00464-5.
7. Ren J#, Yu D#*, Wang J#, Xu K#, Xu Y, Sun R, An P, Li C, Feng G, Zhang Y, Dai X, Zhao H, Wang Z, Han Z, Zhu H, Ding Y, You X, Liu X, Wu M, Luo L, Li Z, Yang YG, Hu Z*, Wei HJ*, Ge L*, Hai T*, Li W*. Generation of immunodeficient pig with hereditary tyrosinemia type 1 and their preliminary application for humanized liver. Cell & Bioscience 2022 Mar 7;12(1):26. doi: 10.1186/s13578-022-00760-3.
8. Guo L^#, Sun X^#, Wang X^#, Liang C^#, Jiang H^#, Gao Q^#, Dai M^#, Qu B, Fang S, Mao Y, Chen Y, Feng G, Gu Q, Wang RR*, Zhou Q*, Li W*. SARS-CoV-2 detection with CRISPR diagnostics. (2020) Cell Discovery 6:34. doi: 10.1038/s41421-020-0174-y.
9. Wang LY^#, Li ZK^#, Wang LB^#, Liu C^#, Sun XH, Feng GH, Wang JQ, Li YF, Qiao LY, Nie H, Jiang LY, Sun H, Xie YL, Ma SN, Wan HF, Lu FL*, Li W*, Zhou Q*. Overcoming Intrinsic H3K27me3 Imprinting Barriers Improves Post-implantation Development after Somatic Cell Nuclear Transfer. (2020) Cell Stem Cell 27(2):315-325.e5. doi: 10.1016/j.stem.2020.05.014.
10. Qu B^#, Wu S^#, Jiao G^#, Zou X^#, Li Z^#, Guo L^#, Sun X, Huang C, Sun Z, Zhang Y, Li H, Zhou Q, Sui R*, Li W*. Treating Bietti crystalline dystrophy in a high-fat diet-exacerbated murine model using gene therapy. (2020) Gene Therapy 27(7-8):370-382.doi: 10.1038/s41434-020-0159-3.
11. Mao J^#, Xu K^#, Han J^#, Feng G, Zhang Y^*, Li W^*. Rapid construction of a whole-genome mutant library by combining haploid stem cells and inducible self-inactivating PiggyBac transposon. (2020) Protein & Cell 11(6):452-457. doi: 10.1007/s13238-020-00702-0.
12. Teng F^#, Guo L^#, Cui T, Wang XG, Xu K, Gao Q, Zhou Q^*, Li W^*. CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity. (2019) Genome Biology 20(1):132. doi: 10.1186/s13059-019-1742-z.
13. Teng F^#, Li J^#, Cui T^#, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou Q^*, Li W^*. Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds. (2019) Genome Biology 20(1):15. doi: 10.1186/s13059-019-1620-8.
14. Li Y^#, Wang L^#, Zhang L^#, He Z^#, Feng G, Sun H, Wang J, Li Z, Liu C, Han J, Mao J, Li P, Yuan X, Jiang L, Zhang Y, Zhou Q^*, Li W^*. Cyclin B3 is required for metaphase to anaphase transition in oocyte meiosis I. (2019) Journal of Cell Biolology 218(5):1553-1563. doi: 10.1083/jcb.201808088.
15. Wang J^#, Wang L^#, Feng G^#, Wang Y^#, Li Y, Li X, Liu C, Jiao G, Huang C, Shi J, Zhou T, Chen Q, Liu Z, Li W^*, Zhou Q^*. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. (2018) Cell 175(7):1887-1901.e18. doi: 10.1016/j.cell.2018.11.039.
16. Teng F^#, Cui T^#, Feng G, Guo L, Xu K, Gao Q, Li T, Li J, Zhou Q, Li W^*. Repurposing CRISPR-Cas12b for mammalian genome engineering. (2018) Cell Discovery 4:63. doi: 10.1038/s41421-018-0069-3.
17. Zhang W^#, Wan H^#, Feng G^#, Qu J^#, Wang J, Jing Y, Ren R, Liu Z, Zhang L, Chen Z, Wang S, Zhao Y, Wang Z, Yuan Y, Zhou Q, Li W^*, Liu G^*, Hu B^*. SIRT6 deficiency results in developmental retardation in cynomolgus monkeys. (2018) Nature 560(7720):661-665. doi: 10.1038/s41586-018-0437-z.
18. Li ZK^#, Wang LY^#, Wang LB^#, Feng GH^#, Yuan XW^#, Liu C, Xu K, Li YH, Wan HF, Zhang Y, Li YF, Li X, Li W^*, Zhou Q^*, Hu BY^*. Generation of Bimaternal and Bipaternal Mice from Hypomethylated Haploid ESCs with Imprinting Region Deletions. (2018) Cell Stem Cell 23(5):665-676.e4. doi: 10.1016/j.stem.2018.09.004.
19. Xu K^#, Yang Y^#, Feng GH^#, Sun BF^#, Chen JQ^#, Li YF, Chen YS, Zhang XX, Wang CX, Jiang LY, Liu C, Zhang ZY, Wang XJ, Zhou Q^*, Yang YG^*, Li W^*. Mettl3-mediated m6A regulates spermatogonial differentiation and meiosis initiation. (2017) Cell Research 27(9):1100-1114. doi: 10.1038/cr.2017.100. (Cover story)
20. Li X^#, Cui XL^#, Wang JQ^#, Wang YK, Li YF, Wang LY, Wan HF, Li TD, Feng GH, Shuai L, Li ZK, Gu Q, Hao J, Wang L, Zhao XY, Liu ZH, Wang XJ, Li W^*, Zhou Q^*. Generation and application of mouse-rat allodiploid embryonic stem cells. (2016) Cell 164(1-2):279-292. doi: 10.1016/j.cell.2015.11.035.
21. Wan HF^#, Feng CJ^#, Teng F^#, Yang SH^#, Hu BY, Niu YY, Xiang P, Fang WZ, Ji WZ, Li W^*, Zhao XY^*, Zhou Q^*. One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system. (2015) Cell Research 25(2):258-261. doi: 10.1038/cr.2014.158.
22. Li W^#, Li X^#, Li TD^#, Jiang MG^#, Wan HF, Luo GZ, Feng CJ, Cui XL, Teng F, Yuan Y, Zhou Q, Gu Q, Shuai L, Sha JH, Xiao YM, Wang L, Liu ZH, Wang XJ, Zhao XY, Zhou Q^*. Genetic modification and screening in rat using haploid embryonic stem cells. (2014) Cell Stem Cell 14(3):404-414. doi: 10.1016/j.stem.2013.11.016.
23. Li W^#, Teng F^#, Li T, Zhou Q^*. Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. (2013) Nature Biotechnology 31(8):684-686. doi: 10.1038/nbt.2652.
24. Li W^#, Shuai L^#, Wan H^#, Dong M^#, Wang M, Sang L, Feng C, Luo GZ, Li T, Li X, Wang L, Zheng QY, Sheng C, Wu HJ, Liu Z, Liu L, Wang L, Wang XJ, Zhao XY^*, Zhou Q^*. Androgenetic haploid embryonic stem cells produce live transgenic mice. (2012) Nature 490(7420):407-411. doi: 10.1038/nature11435.
25. Zhao XY^#, Li W^#, Lv Z^#, Liu L, Tong M, Hai T, Hao J, Guo CL, Ma QW, Wang L, Zeng F^*, Zhou Q^*. iPS cells produce viable mice through tetraploid complementation. (2009) Nature 461(7260):86-90. doi: 10.1038/nature08267.

　　独立之精神，自由之思想。