郭靖涛，中国科学院动物研究所研究员，器官再生与智造全国重点实验室副主任。

2013年本科毕业于北京大学，后赴美国犹他大学攻读博士，毕业后在犹他大学医学院继续深造，进行博士后研究。2020年7月受聘于犹他大学医学院，任准聘助理教授，组建独立课题组，并获多个NIH R01基金资助。2021年12月加入中国科学院动物研究所干细胞与生殖生物学国家重点实验室，任研究组组长。2022年入选国家海外高层次人才计划；获中国科学院战略先导项目、国家重点研发计划、国家自然科学基金面上项目等多个国家级项目资助。以第一作者或通讯作者（含共同）在Cell、 Cell Stem Cell、 Nature Cell Biology、 Nature Aging、 Nature Communications、 Developmental Cell、 Cell Research等期刊上发表研究成果,并受邀在中国、美国、德国、瑞典等多个研究所和高校分享研究成果。长期担任Cell Stem Cell、 Nature Aging、 Nature Communications、 Cell Reports Medicine等期刊独立审稿人。

1.人类精原干细胞的发育与分化调控

发现一类人类特有的精原干细胞类型，提出人类精原干细胞双向可逆的发育模型，为精原干细胞的体外培养及分化提供了候选的靶标分子。

2. 男性生殖细胞和睾丸微环境生理状态的关键事件

系统研究从早期胚胎到青春期和成年期的睾丸发育过程，提出睾丸内生殖细胞和体细胞的发育模型，阐明精原干细胞的完整建立过程，为优化青少年肿瘤生育力保存技术提供新思路。

3. 男性不育和生殖肿瘤的病理机制

研究包括隐睾症在内的男性不育疾病，揭示隐睾患者的精子发生障碍主要源于精原受损，睾丸内生精微环境的功能异常，特别是肥大细胞增多和激活脱颗粒等，为未来临床的精确诊断和针对性治疗策略提供了依据。

4. 基于人工智能的计算生物学工具推动男性不育诊疗

基于全面的多中心大样本量临床数据，引入机器学习模型来预测非梗阻性无精子症(NOA)男性在接受显微睾丸精子提取（micro-TESE）的预后。开发在线工具SpermFinder（https://huggingface.co/spaces/SpermFinder/TESE），预测取精成功率(SRR)，为术前评估和患者知情临床决策提供见解。

　（#共同第一作者, *通讯作者）

1. Cui L^#, Nie X^#, Guo YX^#, Ren P, Guo YF, Wang X, Li R, Hotaling JM, Cairns BR*, Guo J*. Single-cell transcriptomic atlas of human testis across the reproductive lifespan. Nature Aging. 2025 Mar 3. (On Line)
2. Jiang Q, Cui L, Nie X, Cai H, Zhang W, Lu X, Guo Y, Hotaling JM, Cairns BR, Wang X*, Guo J*. A single-cell transcriptome atlas characterizes the immune landscape of human testes during aging. Aging Cell. 2025 Mar 6;e70032.
3. Cui L^#, Lin S^#, Yang X^#, Xie X^#, Wang X^#, He N^#, Yang J, Zhang X, Lu X, Yan X, Guo Y, Zhang B, Li R, Miao H, Ji M, Zhang R, Yu L*, Xiao Z*, Wei Y*, Guo J*. Spatial transcriptomic characterization of a Carnegie stage 7 human embryo. Nat Cell Biol. 2025 Feb;27(2):360-369.
4. Wang X^#, Cheng L^#, Lu X^#, Jin H^#, Cui L, Guo Y, Guo J*, Xu EY*. Cross-species comparative single-cell transcriptomics highlights the molecular evolution and genetic basis of male infertility. Cell Rep. 2024 Dec 30;44(1):115118.
5. Wang X^#, Wang Y^#, Wang Y^#, Guo Y^#, Zong R, Hu S, Yue J, Yao J, Han C*, Guo J*, Zhao J*. Single-cell transcriptomic and cross species comparison analyses reveal distinct molecular changes of porcine testes during puberty. Commun Biol. 2024 Nov 9;7(1):1478.
6. Wang X^#, Liu Q^#, Zhuang Z^#, Cheng J, Zhang W, Jiang Q, Guo Y, Li R, Lu X, Cui L, Weng J, Tang Y, Yue J, Gao S, Hong K, Qiao J, Jiang H*, Guo J*, Zhang Z*. Decoding the pathogenesis of spermatogenic failure in cryptorchidism through single-cell transcriptomic profiling. Cell Rep Med. 2024 Sep 17;5(9):101709.
7. Cui Y, Harteveld F, Ba Omar HAM, Yang Y, Bjarnason R, Romerius P, Sundin M, Norén Nyström U, Langenskiöld C, Vogt H, Henningsohn L, Frisk P, Vepsäläinen K, Petersen C, Mitchell RT, Guo J, Alves-Lopes JP, Jahnukainen K, Stukenborg JB. Prior exposure to alkylating agents negatively impacts testicular organoid formation in cells obtained from childhood cancer patients. Hum Reprod Open. 2024 Aug 13;2024(3):hoae049.
8. Xiao Z^#, Cui L^#, Yuan Y^#, He N^#, Xie X, Lin S, Yang X, Zhang X, Shi P, Wei Z, Li Y, Wang H, Wang X*, Wei Y*, Guo J*, Yu L*. 3D reconstruction of a gastrulating human embryo. Cell. 2024 May 23;187(11):2855-2874.e19.
9. Lu X^#, Luo Y^#, Nie X^#, Zhang B, Wang X, Li R, Liu G, Zhou Q, Liu Z, Fan L, Hotaling JM, Zhang Z*, Bo H*, Guo J*. Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment. Nat Commun. 2023 Dec 20;14(1):8462.
10. Wamaitha SE^#, Nie X^#, Pandolfi EC, Wang X, Yang Y, Stukenborg JB, Cairns BR, Guo J*, Clark AT*. Single-cell analysis of the developing human ovary defines distinct insights into ovarian somatic and germline progenitors. Dev Cell. 2023 Oct 23;58(20):2097-2111.e3.
11. Matilionyte G, Forsyth G, Guo J, Rimmer MP, Hermann BP, Anderson RA, Mitchell RT. Granulocyte-colony stimulating factor does not prevent in vitro cisplatin-induced germ cell reduction in immature human and mouse testis.  BMC Cancer. 2023 Mar 16 ;23(1):251.
12. Nie X, Munyoki SK, Sukhwani M, Schmid N, Missel A, Emery BR, DonorConnect, Stukenborg JB, Mayerhofer A, Orwig KE, Aston KI, Hotaling JM, Cairns BR*, Guo J*. Single-Cell Analysis of Human Testis Aging and Correlation with Elevated Body Mass Index. Dev Cell. 2022 May 9;57(9):1160-1176.e5.
13. Wang X, Cairns BR*, Guo J*. When spermatogenesis meets human aging and elevated body mass. Life Med. 2022 Jul 9;1(3):267-269.
14. Mahyari E^#, Guo J^#*, Lima AC, Lewinsohn DP, Stendahl AM, Vigh-Conrad KA, Nie X, Nagirnaja L, Rockweiler NB, Carrell DT, Hotaling JM, Aston KI, Conrad DF*. Comparative single-cell analysis of biopsies clarifies pathogenic mechanisms in Klinefelter syndrome. Am J Hum Genet. 2021 Oct 7;108(10):1924-1945.
15. Patel DP, Punjani N, Guo J, Alukal JP, Li PS, Hotaling JM*. The impact of SARS-CoV-2 and COVID-19 on male reproduction and men's health. Fertil Steril. 2021 Apr;115(4):813-823.
16. Guo J*, Sosa E, Chitiashvili T, Nie X, Rojas EJ, Oliver E; DonorConnect; Plath K, Hotaling JM, Stukenborg JB, Clark AT*, Cairns BR*. Single-cell analysis of the developing human testis reveals somatic niche cell specification and fetal germline stem cell establishment. Cell Stem Cell. 2021 Apr 1;28(4):764-778.e4.
17. Tharmalingam MD, Matilionyte G, Wallace WHB, Stukenborg JB, Jahnukainen K, Oliver E, Goriely A, Lane S, Guo J, Cairns B, Jorgensen A, Allen CM, Lopes F, Anderson RA, Spears N, Mitchell RT. Cisplatin and carboplatin result in similar gonadotoxicity in immature human testis with implications for fertility preservation in childhood cancer. BMC Med. 2020 Dec 4 ;18(1):374.
18. Murphy PJ^#*, Guo J^#, Jenkins TG, James ER, Hoidal JR, Huecksteadt T, Broberg DS, Hotaling JM, Alonso DF, Carrell DT, Cairns BR*, Aston KI*. NRF2 loss recapitulates heritable impacts of paternal cigarette smoke exposure. PLoS Genet. 2020 Jun 10;16(6):e1008756.
19. Guo J, Nie X, Giebler M, Mlcochova H, Wang Y, Grow EJ; DonorConnect; Kim R, Tharmalingam M, Matilionyte G, Lindskog C, Carrell DT, Mitchell RT, Goriely A, Hotaling JM, Cairns BR*. The Dynamic Transcriptional Cell Atlas of Testis Development during Human Puberty. Cell Stem Cell. 2020 Feb 6;26(2):262-276.e4.
20. Guo J*, Cairns BR. Isolation and Enrichment of Spermatogonial Stem Cells From Human Testis Tissues. Curr Protoc Stem Cell Biol. 2019 Jun;49(1):e77.
21. Guo J, Grow EJ, Mlcochova H, Maher GJ, Lindskog C, Nie X, Guo Y, Takei Y, Yun J, Cai L, Kim R, Carrell DT, Goriely A, Hotaling JM, Cairns BR*. The adult human testis transcriptional cell atlas. Cell Res. 2018 Dec;28(12):1141-1157.
22. Guo J, Grow EJ, Yi C, Mlcochova H, Maher GJ, Lindskog C, Murphy PJ, Wike CL, Carrell DT, Goriely A, Hotaling JM, Cairns BR*. Chromatin and Single-Cell RNA-Seq Profiling Reveal Dynamic Signaling and Metabolic Transitions during Human Spermatogonial Stem Cell Development. Cell Stem Cell. 2017 Oct 5;21(4):533-546.e6.