姓  名: 郑 瑚
学  科: 基因调控与治疗
电话/传真:  / 
电子邮件: albert.cheng@ioz.ac.cn
通讯地址:
更多信息: 郑瑚研究组     

简历介绍:

郑瑚,研究员,博士生导师, 研究组组长。2014年博士毕业于美国麻省理工学院计算与系统生物学系,师从Christopher Burge和Rudolf Jaenisch教授。博士期间,他研究的课题广泛,包括重编程、干细胞分化、癌症转移及红细胞生成中的表观遗传和RNA选择性剪接机制,以及CRISPR技术的开发利用等。 主要研究成果有:将H3K27ac鉴定为活性增强子的标志;分析了癌症转移以及红细胞生成中的RNA选择性剪接,并鉴定了调节这些过程的剪接因子;构建了CRISPR-on基因激活工具。 2014年从麻省理工学院毕业后,他加入了美国杰克逊实验室,成为首批杰克逊学者之一,继续致力于改进CRISPR / Cas技术。 自2015年7月始,他在杰克逊实验室及其后于亚利桑那州立大学建立独立实验室,开发CRISPR/Cas和其他合成生物学的技术,譬如Casilio、CASFx、CREST、TALEsense系统等。郑瑚博士在Cell 、Nature Biotechnology、Cell Research、Cancer Discovery、Cell Stem Cell、 Blood、及PNAS等高影响因子杂志发表文章40余编,总引用次数超过两万(Google Scholar) 。

研究领域:

1. 开发基因组、表观基因组和转录组编辑和传感的新方法。

2. 开发三维基因组结构成像技术。

3. 研究发育和疾病中的三维基因组、表观基因组和转录组的变化。

4. 开发新型基因疗法。

5. 建立人工 DNA/RNA 结合蛋白和引导 RNA 设计的计算模型。

社会任职:

获奖及荣誉:

承担科研项目情况:

代表论著:

*通讯, #共同一作

  1. Liu, Z., Jillette N., Robson, P., Cheng, A.W.* (2023) Simultaneous multifunctional transcriptome engineering by CRISPR RNA scaffold. Nucleic Acid Research gkad547 doi: 10.1093/nar/gkad547. IF=19.16
  2. Clow, P.A., Du, M., Jillette, N., Taghbalout, A., Zhu, J.J.*, Cheng, A.W.* (2022) CRISPR-mediated multiplexed live cell imaging of nonrepetitive genomic loci with one guide RNA per locus. Nature Communications 13:1871. doi: 10.1038/s41467-022-29343-z. IF=17.694
  3. Yi, E., Gujar, A.D., Guthrie, M., Kim, H., Zhao, D., Johnson K.C., Amin, S.B., Costa, M.L., Yu, Q., Das, S., Jillette, N., Clow, P.A., Cheng, A.W.*, Verhaak, R.G.W.* (2022) Live-cell imaging shows uneven segregation of extrachromosomal DNA elements and transcriptionally active extrachromosomal DNA hubs in cancer. Cancer Discovery doi: 10.1158/2159-8290.CD-21-1376 (co-corresponding) IF=39.397
  4. Zhu, J.J., Cheng, A.W.* (2022) JACKIE: Fast enumeration of genome-wide single- and multi-copy CRISPR target sites and their off-target numbers. The CRISPR Journal doi: 10.1089/crispr.2022.0042 IF=4.321
  5. Du, M.#, Jillette, N.#, Zhu, J.J., Li, S., Cheng, A.W.* (2020) CRISPR Artificial Splicing Factors. Nature Communications 11:2973. doi: 10.1038/s41467-020-16806-4 IF=17.694
  6. Zhu, J.J., Jillette, N., Li X., Cheng, A.W.*, Lau C.C.* (2020) C11orf95-RELA Reprograms 3D Epigenome in Supratentorial Ependymoma. Acta Neuropathologica doi: 10.1007/s00401-020-02225-8 (co-corresponding) IF=21.534
  7. Taghbalout, A., Du, M., Jillette, N., Rosikiewicz, W. Rath, A., Heinen, C., Li, S., Cheng, A.W.* (2019) Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways. Nature Communications 10:4296. doi:10.1038/s41467-019-12339-7 IF=17.694
  8. Jillette, N.#., Du, M.#, Zhu, J.J., Cardoz, P., Cheng, A.W.* (2019) Split Selectable Markers. Nature Communications 10:4968. doi: 10.1038/s41467-019-12891-2 IF=17.694
  9. Cheng, A.W.*#, Jillette, N.#, Lee, P., Plaskon, D., Fujiwara, Y., Wang, W., Taghbalout, A., Wang, H.* (2016) Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling. Cell Research 26:254–257. doi: 10.1038/cr.2016.3 PMID:26768771 (co-corresponding) IF=46.3
  10. Cheng, A.W.#, Shi, J.#, Wong, P.#, Luo, K.L., Trepman, P., Wang, E.T., Choi, H., Burge, C.B., Lodish, H.F.* (2014) Muscleblind-like 1 (Mbnl1) regulates pre-mRNA alternative splicing during terminal erythropoiesis. Blood doi: 10.1182/blood-2013-12-542209 PMID: 24869935 IF=25.48
  11. Cheng, A.W.#, Wang, H.#, Yang, H, Shi, L., Katz, Y., Theunissen, T.W., Rangarajan, S., Shivalila, C.S., Dadon, D.B., Jaenisch, R.* (2013) Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Research 23(10):1163-71 PMID: 23979020 IF=46.3
  12. Yang, H.#, Wang, H.#, Shivalila, C.S.#, Cheng, A.W., Shi, L., Jaenisch, R.* (2013). One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell 154(6):1370-9 PMID: 23992847
  13. Wang, H.#, Yang, H.#, Shivalila, C.S.#, Dawlaty, M.M., Cheng, A.W., Zhang, F., Jaenisch, R.* (2013). One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell 153(4):910-8 PMID: 23643243
  14. Buganim, Y.#, Faddah D.A.#, Cheng, A.W., Itskovich, E., Markoulaki, S., Gantz, K., Klemm S.L., van Oudenaarden A., Jaenisch, R.* (2012) Single-Cell Expression Analyses during Cellular Reprogramming Reveal an Early Stochastic and a Late Hierarchic Phase. Cell 150(6):1209-22 PMID: 22980981
  15. Shapiro, I.M.#, Cheng, A.W.#, Flytzanis, N.C., Balsamo, M., Condeelis, J.S., Oktay, M.H., Burge, C.B.*, Gertler, F.B.* (2011) An EMT-driven alternative splicing program occurs in human breast cancer and modulates cellular phenotype. PLoS Genet. 7(8):e1002218 PMID: 21876675 (co-first) IF=6.02
  16. Creyghton, M.P.#, Cheng A.W.#, Welstead, G.G., Kooistra, T., Carey, B.W., Steine, E.J., Hanna, J., Lodato, M.A., Frampton, G.M., Sharp, P.A., Boyer, L.A., Young, R.A.*, Jaenisch, R.* (2010) Histone H3K27ac separates active from poised enhancers and predicts developmental state. Proc. Natl. Acad. Sci. U.S.A. 107(50):21931-6 PMID: 21106759 (co-first) IF=12.78

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